Abstract:
Background: Antimicrobial resistance represents a growing global health concern, with foodborne bacteria acting as important reservoirs in the transmission pathway. Specific background: Escherichia coli capable of producing Extended Spectrum Beta Lactamase has been frequently detected in animal-derived foods, particularly beef sold in traditional markets where sanitation practices are often inadequate. Knowledge gap: Despite numerous studies on livestock and slaughterhouses, evidence describing the prevalence pattern of Extended Spectrum Beta Lactamase producing Escherichia coli in beef at the traditional market level remains limited, especially in Surabaya. Aims: This study aimed to describe the prevalence pattern of Extended Spectrum Beta Lactamase producing Escherichia coli in beef sold in traditional markets. Results: A descriptive cross-sectional study was conducted on fifty beef samples collected from multiple vendors. Identification using MacConkey agar and confirmation with the Double Disk Synergy Test showed that 29 samples (58%) were positive for Extended Spectrum Beta Lactamase producing Escherichia coli, with variation across vendors. Novelty: This study provides direct phenotypic confirmation of Extended Spectrum Beta Lactamase producing Escherichia coli in beef from traditional market settings. Implications: The findings emphasize the need for routine microbiological monitoring and improved hygiene practices in traditional meat distribution to support food safety and public health protection.
Highlights
Extended Spectrum Beta Lactamase producing Escherichia coli detected in more than half of beef samples
Prevalence pattern varied among traditional market vendors
Traditional market meat handling presents substantial food safety challenges
Keywords
Beef, Escherichia Coli, Extended Spectrum Beta Lactamase, Traditional Markets, Antimicrobial Resistance
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References
[1] Akyun Rozaqi Syah Putra 1), M. H. E. 2), S. K. 2), S. 2), W. T. 2), A. T. S. E. 2). (2019). Akyun Rozaqi. dkk. Identifikasi Bakteri Eschericia coli Penghasil Extended Spectrum β-Lactamase.
[2] Barreiro, C., Albano, H., Silva, J., & Teixeira, P. (2013). Role of Flies as Vectors of Foodborne Pathogens in Rural Areas. ISRN Microbiology, 2013, 1–7. https://doi.org/10.1155/2013/718780
[3] Biutifasari, V. (2018). Extended Spectrum Beta-Lactamase (ESBL). In LITERATURE STUDY Oceana Biomedicina Journal (Vol. 1, Issue 1). www.wiley.com
[4] Blaak, H., Hamidjaja, R. A., Van Hoek, A. H. A. M., De Heer, L., De Roda Husman, A. M., & Schets, F. M. (2013). Detection of extended-spectrum beta-lactamase (ESBL)-producing escherichia coli on flies at poultry farms. Applied and Environmental Microbiology, 80(1), 239–246. https://doi.org/10.1128/AEM.02616-13
[5] Farmasi, M., Farmakologi, D., Subhan, M., Sibadu, A., Djide, M. N., Massi, M. N., & Mus, N. M. (2023). Pseudomonas aeruginosa di RSUP DR. WAHIDIN SUDIROHUSODO MAKASSAR. Original Article MFF, 27(1), 1–4. https://doi.org/10.20956/mff.v27i1.13574
[6] Ghafourian, S., Sadeghifard, N., Soheili, S., & Sekawi, Z. (2014). Extended spectrum beta-lactamases: Definition, classification and epidemiology. Current Issues in Molecular Biology, 17(1), 11–22. https://doi.org/10.21775/cimb.017.011
[7] Gilang Prasaja, A., Wulandari, E., Tyas Kusuma Anggaeni, T., Sukmawinata, E., Studi Kedokteran Hewan, P., Kedokteran, F., Padjadjaran, U., Riset Veteriner, P., Riset Kesehatan, O., & Riset dan Inovasi Nasioal, B. (2023). ACTA VETERINARIA INDONESIANA Escherichia coli Penghasil Extended-spectrum Β-Lactamases yang Diisolasi pada Peternakan Broiler-Ikan Terintegrasi (Extended-Spectrum β-Lactamases producing Escherichia coli isolated from Integrated Broiler-Fish Farming). http://www.journal.ipb.ac.id/indeks.php/actavetindones
[8] Guo, S., Aung, K. T., Leekitcharoenphon, P., Tay, M. Y. F., Seow, K. L. G., Zhong, Y., Ng, L. C., Aarestrup, F. M., & Schlundt, J. (2021). Prevalence and genomic analysis of ESBL-producing Escherichia coli in retail raw meats in Singapore. Journal of Antimicrobial Chemotherapy, 76(3), 601–605. https://doi.org/10.1093/jac/dkaa461
[9] Hartanto, W. A., Hendrayana, M. A., & Budayanti, N. N. S. (2023). Identifikasi kontaminasi bakteri E. coli 0157 pada daging sapi di pasar tradisional kota Denpasar. Intisari Sains Medis, 14(3), 1287–1293. https://doi.org/10.15562/ism.v14i3.1891
[10] Jafar, M., Martani, N. S., Jabal, A. R., Furtuna, D. K., & Ratnasari, A. (2024). Cemaran Bakteri Escherichia coli Dan Salmonella sp. Pada Daging Sapi Di Pasar Tradisional Kota Palangka Raya. Jurnal Media Analis Kesehatan, 15(1), 46–57. https://doi.org/10.32382/jmak.v15i1.391
[11] Kanah Arieska, P., Herdiani, N., Studi Ilmu Kesehatan Masyarakat, P., Kesehatan, F., & Nahdlatul Ulama Surabaya Alamat, U. (2018). PEMILIHAN TEKNIK SAMPLING BERDASARKAN PERHITUNGAN EFISIENSI RELATIF (Vol. 6, Issue 2). http://jurnal.unimus.ac.id
[12] Maghfiroh, L., Helmi Effendi, M., Tyasningsih, W., Witaningrum, A. M., Bernard, A. N., & Helmi, M. (2024). Identification of BlaTEM Gene Encode Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli Isolated from Fresh Beef. In Full Length Research Article Advancements in Life Sciences-International Quarterly Journal of Biological Sciences Advancements in Life Sciences | www.als-journal.com (Vol. 11). www.als-journal.com
[13] Mandujano-Hernández, A., Martínez-Vázquez, A. V., Paz-González, A. D., Herrera-Mayorga, V., Sánchez-Sánchez, M., Lara-Ramírez, E. E., Vázquez, K., de Jesús de Luna-Santillana, E., Bocanegra-García, V., & Rivera, G. (2024). The Global Rise of ESBL-Producing Escherichia coli in the Livestock Sector: A Five-Year Overview. In Animals (Vol. 14, Issue 17). Multidisciplinary Digital Publishing Institute (MDPI). https://doi.org/10.3390/ani14172490
[14] Marbella, S., Langgar, C., Urias, M., Sanam, E., & Detha Detha, A. I. R. (2021). PREVALENSI Escherichia coli PADA DAGING SAPI DI RUMAH POTONG HEWAN OEBA KOTA KUPANG. http://ejurnal.undana.ac.id/jvnVol.4No.1
[15] Masrianto, F. dan A. (2013). UJI RESIDU ANTIBIOTIK PADA DAGING SAPI YANG DIPASARKAN DI PASAR TRADISIONAL KOTA BANDA ACEH The Examination of Antibiotic Residue on Beef Sold at Traditional Market in Banda Aceh.
[16] Nanda, K. B., Kuntaman, K., & Indrachan, W. (2023). Pola Kejadian Escherichia coli Penghasil ESBL pada Caecum Ayam Broiler di Pasar Tradisional. In Calvaria Medical Journal.
[17] Normaliska, R., Bachrum Sudarwanto, M., Latif, H., Besar Karantina Pertanian Tanjung Priok, B., Karantina Pertanian, B., Studi Kesehatan Masyarakat Veteriner Sekolah Pascasarjana IPB, P., Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner, D., & Kedokteran Hewan, F. (2019). Pola Resistensi Antibiotik pada Escherichia coli Penghasil ESBL dari Sampel Lingkungan di RPH-R Kota Bogor (Antibiotic Resistance of ESBL-Producing Escherichia coli from Environmental Samples in Bogor Slaughterhouse). ACTA VETERINARIA INDONESIANA, 7(2), 42–48. http://www.journal.ipb.ac.id/indeks.php/actavetindones
[18] Padang, U. N., Fadhilah Ilahi, N., Ananta, N. L., Advinda, L., & Kes, M. (2021). Prosiding SEMNAS BIO 2021 Kualitas Mikrobiologi Daging Sapi dari Pasar Tradisional.
[19] Pisestyani, H., Lukman, D. W., Latif, H., & Sudarwanto, M. (2023). Keberadaan Escherichia coli Extended Spectrum β-lactamase Resistan Antibiotik di Peternakan Sapi Perah Cijeruk, Bogor. Jurnal Veteriner, 23(4), 505–514. https://doi.org/10.19087/jveteriner.2022.23.4.505
[20] Rahmawati, L., Sasongkowati, R., Anggraini, A. D., Adam, D., Laboratorium, J. T., Poltekkes, M., Surabaya, K., Kesehatan, J., & Poltekkes, L. (2022). coli PENGHASIL Extended Spectrum Beta-Lactamase (ESBL) MENGGUNAKAN METODE PCR PADA SAMPEL AIR BERSIH (Vol. 20, Issue 02).
[21] Siagian, G., Pd, S., & Si, M. (2020). TAKSONOMI HEWAN. www.penerbitwidina.com
[22] Sri Rahayu, R., & Kesehatan Masyarakat UTU Kab Aceh Barat, F. (2022). IDENTIFIKASI BAKTERI ESCHERICIA COLI PADA DAGING SAPI DI PASAR BINA USAHA MEULABOH. Jurnal Mahasiswa Kesehatan Masyarakat, 2(2).
[23] Studi Biologi, P. (2016). ISOLASI Escherichia coli PADA DAGING SAPI SEGAR YANG DIPEROLEH DARI BEBERAPA PASAR TRADISIONAL DI PEKANBARU Elsie, Israwati Harahap. In Jurnal Photon (Vol. 7, Issue 1).
[24] Suherman, D. A., Sudarnika, E., & Purnawarman, T. (2023). Resistansi Penisilin terhadap Escherichia coli pada Susu Segar yang berasal dari Koperasi Ternak Sapi Cianjur Utara (KPSCU), Jawa Barat. Jurnal Sain Veteriner, 41(2), 170. https://doi.org/10.22146/jsv.83050
[25] Theresia Ika Purwaningsih dan Kristoforus Wilson Kia. (2018). Identifikasi dan recording sapi perah di peternakan biara novisiat craletian benlutu, timor tengah selatan.
[26] Wardhana, D. K., Safitri, D. A., Annisa, S., Harijani, N., Estoepangestie, A. T. S., & Maghfiroh, L. (2021). Prevalence of Extended-Spectrum Beta-Lactamases in producing Escherichia coli in beef sold in traditional markets in Surabaya, Indonesia. Biodiversitas, 22(5), 2789–2793. https://doi.org/10.13057/biodiv/d220542
[27] Wetzker, W., Pfeifer, Y., Wolke, S., Haselbeck, A., Leistner, R., Kola, A., Gastmeier, P., & Salm, F. (2019). Extended-spectrum beta-lactamase (ESBL)-producing escherichia coli isolated from flies in the urban center of Berlin, Germany. International Journal of Environmental Research and Public Health, 16(9). https://doi.org/10.3390/ijerph16091530
[28] Wicaksono. (2016). 4. Chapter 2.
[29] Widhi, A. P. K. N., & Saputra, I. N. Y. (2021). Residu Antibiotik Serta Keberadaan Escherichia Coli Penghasil ESBL pada Daging Ayam Broiler di Pasar Kota Purwokerto. Jurnal Kesehatan Lingkungan Indonesia, 20(2), 137–142. https://doi.org/10.14710/jkli.20.2.137-142
[30] Winiati P. Rahayu, S. N. E. K. (2018). B4_Buku 2.
[31] Yanestria, S. M., Dameanti, F. N. A. E. P., Musayannah, B. G., Pratama, J. W. A., Witaningrum, A. M., Effendi, M. H., & Ugbo, E. N. (2022). Antibiotic resistance pattern of Extended-Spectrum β-Lactamase (ESBL) producing Escherichia coli isolated from broiler farm environment in Pasuruan district, Indonesia. Biodiversitas, 23(9), 4460–4465. https://doi.org/10.13057/biodiv/d230911