Uji Aktivitas Antidiabetes Ekstrak Bonggol Nanas (Ananas comusus L.) pada Tikus yang Di induksi Aloksan

DOI: https://doi.org/10.21070/medicra.v1i1.1473

Author (s)


(1) * Ayu Rochmawati   (Universitas Muhammadiah Sidoarjo)  
        Indonesia
(2)  Syahrul Ardiansyah   (Universitas Muhammadiyah Sidoarjo)  
        Indonesia
(*) Corresponding Author

Abstract


This research aims to knowing about decrease glucose levels in rat induced alloxan with the provision of pineapple stem extract (Ananas comusus L.). Previous studies have shown that bromelin can significantly reduce CD4+ lymphocytes, which are included in inflammatory diseases. One of the inflammatory diseases is diabetes mellitus. Blood glucose levels can be lowered by pineapple extract as it contains bromelin, and bromelin most widely found on the stem. The test animals used white rat strain wistar (Rattus norvegicus L.) with weight is 250-300 gram acclimated for seven days. The research was divided  six treatment groups: positive control, negative control, P1 (25% concentration), P2 (50% concentration), P3 (75% concentration), P4 (100% concentration). After treatment for rat in category or sampel was administered pineapple stem extract for 14 days after that experiment status blood glucose level. The results showed that rat had  increased (hyperglicemic) after alloxan induced,and also in blood glucose levels in all treatment. the biggest decrease was in P4 is 44 mgdl. The statistic of Kruskal-Wallis test showed that the decrease of rat blood glucose concentration at various concentration of pineapple extract influence  signifificantly. According to the result  that conclusion is  the difference of pineapple extract concentration (25%, 50%, 75%, 100%) influence for decrease blood glucose levels and decreasing blood glucose level after 14 days, but in this  time blood glucose  not returned as normal conditions.

 

 



Keywords

alloxan; antidiabetic; pineapple extract; rat (Rattus norvegicus L.)



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References


Bala, M., Ismail, N. A., Mel, M., Jami, M. S., Saleh, H. M., & Amid, A. (2012). Bromelain Production: Current Trends And Perspective. Archives Des Sciences, (65)11, 369-399. Retrieved from http://irep.iium.edu.my/28364/1/Bromelain_review.pdf

Borrelli, F., Capasso, R., Severini, B., Fiorino, F., Aviello, G., ... Izzo, A. A. (2011). Inibitory Effect of Bromelin, a Cysteine Protease Derived from Pineapple Stem (Ananas comusus), on Intestinal Motility in Mice. Neurogastroenterol Motil, 23(8), 745-e331. doi: 10.1111/j.1365-2982.2011.01735.x

Darmono. 2007. Status Glikemi dan Komplikasi Vaskuler Diabetes Mellitus. Djokomoeljanto R, Darmono, Suhartono T, Pemayun Tgd (Eds). Kongres Nasional V Persatuan Diabetes Indonesia (hal. 57-68). Semarang.

Dinas Kesehatan Republik Indonesia. (2008). Pedoman Pelayanan Kefarmasian Dirumah/ Home Pharmacy Care (hal. 16-20). Jakarta: Depkes RI.

Ladhams, A., Scarnto, P., & Engwerda, C. (1999). Bromelain from Pineapple Stems Proteolytically Blocks Activation of Extracelluler Regulated Kinase-2 in T Cells. J Immunol, 163(5), 2568-75. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/10452995

Pavan, R., Jain, S., Shraddha, & Kumar, A. (2012). Properties And Therapeutic Application Of Bromelain: A Review. Biotechnology Research International, Vol 12, 1-6. doi: 10.1155/2012/976203

Schwinghammer, L. (2009). Diabetes Mellitus In Dipro, Et Al, Pharmacotherapy Handbook 7th Edition (pp. 210-226). USA: The Mc-Graw Hill.

Smeltzer, Suzanne, C., Bare, & Brenda, G. (2002). Buku Ajar Keperawatan Medikal Bedah Brunner Dan Suddarth. Jakarta: EGC.

Suyono, S. (2007). Patofisiologi Diabetes Mellitus Dalam Penatalaksanan Diabetes Mellitus Terpadu. Jakarta: Balai Penerbit FKUI.

Tochi, B.N., Wang, Z., Ying Xu, S., & Zang, W. (2008). Theraupetik application of pineapple protase (bromelin): A Review. Pakistan journal of nutrition, 7(40), 513-520. Retrieved from http://docsdrive.com/pdfs/ansinet/pjn/2008/513-520.pdf

World Health Organization. (2008). Prevention of Diabetes Mellitus. Geneva: Technical report series 844.

Yuriska, F.A. (2009). Efek Aloksan Terhadap Kadar Glukosa Darah Tikus Wistar. Skripsi. Fakultas Kedokteran. Universitas Diponegoro. Semarang.


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10.21070/medicra.v1i1.1473




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